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KMID : 0381120110330060727
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Genes and Genomics
2011 Volume.33 No. 6 p.727 ~ p.733
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Development of expressed sequence tag derived-simple sequence repeats in the genus Lilium
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Lee Sung-Il
Park Kyong-Cheul
Song Ye-Su
Son Jae-Han
Kwon Soon-Jae
Na Jong-Kuk
Kim Jong-Hwa
Kim Nam-Soo
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Abstract
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Although lily is the second largest flower crop in cutting flower commodity, only six simple sequence repeats SSRs have been reported. Thus, we developed expressed sequence tag derived-SSRs (EST-SSRs) for the Lilium genus. Among 2,235 unique ESTs, 754 ESTs contained SSR motifs, among which 165 ESTs were amenable to primer design. Among these 165 EST-SSRs, 131 EST-SSRs showed amplification in at least one Lilium species, and 76 EST-SSRs showed amplification in at least nine species. Of the 76 EST-SSRs, 47 showed amplification in all Lilium species analyzed. Using 10 breeding lines, we selected 21 EST-SSRs that had the highest number of alleles and polymorphism information content. The polymorphism information content values of these selected EST-SSRs ranged from 0.49 to 0.94 with an average of 0.76, which are higher than other plant species. The phylogenetic dendrogram derived from the amplification profiles of the 21 high polymorphic EST-SSRs was congruent with the genetic background of the 84 selected lily accessions and hybrids, which are available in commerce. Thus, the developed EST-SSRs will be very useful in germplasm management, genetic diversity analysis, cultivar finger printing, and molecular breeding in the lily.
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KEYWORD
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lily, EST-SSR, Polymorphism content information, Cross-species SSR amplification
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